Inhibitory Effect of Rhodomyrtus tomentosa Methanolic Leaves Extract on P-selectin Platelet Aggregation of ABO Blood Group by ELISA Method

Evana Kamarudin, Nur Fadhillah Megat Ismail, Siti Norul Fatin Ismail


In light of discovering the new antiplatelet from the natural source, this study was undertaken to determine the inhibitory effect of Rhodomyrtus tomentosa methanolic leaves extracts on P-selectin platelet aggregation of the ABO blood group. Whole blood samples from each blood group were collected tested with different concentration of leaves extract by using ELISA method. The inhibition was considered to occur if the values of concentration P selectin detected below 1.56 ng/ml. Instead of inhibiting the P-selectin expression as antiplatelet, Rhodomyrtus tomentosa accelerates the P-selectin expression as an agonist in a dose-dependent manner. In conclusion, no antiplatelet effects were seen.

Keywords: P-selectin, Rhodomyrtus tomentosa, platelet aggregation, ABO Blood Group

eISSN: 2398-4287 © 2019. The Authors. Published for AMER ABRA cE-Bs by e-International Publishing House, Ltd., UK. This is an open access article under the CC BYNC-ND license ( Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians) and cE-Bs (Centre for Environment-Behaviour Studies), Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.

Full Text:



Abd Hamid, H., Mutazah, R., Yusoff, M. M., Abd Karim, N. A., & Abdull Razis, A. F. (2017). Comparative analysis of antioxidant and antiproliferative activities of Rhodomyrtus tomentosa extracts prepared with various solvents. Food and Chemical Toxicology, 108, 451–457.

Aziz, Z. A., Lee, Y. Y. L., Ngah, B. A., Sidek, N. N., Looi, I., Hanip, M. R., & Basri, H. B. (2015). Acute Stroke Registry Malaysia, 2010-2014: Results from the National Neurology Registry. Journal of Stroke and Cerebrovascular Diseases, 24(12), 2701–2709.

Bachmair, E. M., Ostertag, L. M., Zhang, X., & De Roos, B. (2014). Dietary manipulation of platelet function. Pharmacology and Therapeutics, 144(2), 97–113.

Blann, A. D. (2014). Soluble P-selectin : The next step. Thrombosis Research, 133(1), 3–4.

Cicerale, S., Lucas, L. J., & Keast, R. S. J. (2012). Antimicrobial, antioxidant and anti-inflammatory phenolic activities in extra virgin olive oil. Current Opinion in Biotechnology, 23(2), 129–135.

El Haouari, M., López, J. J., Mekhfi, H., Rosado, J. A., & Salido, G. M. (2007). Antiaggregant effects of Arbutus unedo extracts in human platelets. Journal of Ethnopharmacology, 113(2), 325–331.

El Haouari, M., & Rosado, J. A. (2008). Platelet signalling abnormalities in patients with type 2 diabetes mellitus: A review. Blood Cells, Molecules, and Diseases, 41(1), 119–123.

Faggio, C., Sureda, A., Morabito, S., Sanches-silva, A., Mocan, A.,

Fazel, S., & Mohammad, S. (2017). Flavonoids and platelet aggregation : A brief review, 807(October 2016), 91–101.

Goldstein, L. B., Bushnell, C. D., Adams, R. J., Appel, L. J., Braun, L. T., Chaturvedi, S., … Pearson, T. A. (2011). Guidelines for the primary prevention of stroke: A Guideline for Healthcare Professionals from the American Heart Association/American Stroke Association. Stroke, 42(2), 517–584.

Hasibuan, R., Ilyas, S., & Hanum, S. (2015). Effect of leaf extract haramonting (Rhodomyrtus tomentosa) to lower blood sugar levels in mice induced by alloxan. International Journal of PharmTech Research, 8(6), 284–291.

Ho, J. A., Jou, A. F., Wu, L., & Hsu, S. (2012). Development of an immunopredictor for the evaluation of the risk of cardiovascular diseases based on the level of soluble P-selectin. Methods, 56(2), 223–229.

Lai, T. N. H., Herent, M. F., Quetin-Leclercq, J., Nguyen, T. B. T., Rogez, H., Larondelle, Y., & André, C. M. (2013). Piceatannol, a potent bioactive stilbene, as major phenolic component in Rhodomyrtus tomentosa. Food Chemistry, 138(2–3), 1421–1430.

Lalko, C. C., Deppe, E., Ulatowski, D., Lutgen, A., Hart, A. P., Patton, E. A., … Darien, B. J. (2003). Equine platelet CD62P (P-selectin) expression: A phenotypic and morphologic study. Veterinary Immunology and Immunopathology, 91(2), 119–134.

Mordmuang, A., & Voravuthikunchai, S. P. (2015). Research in Veterinary Science Rhodomyrtus tomentosa ( Aiton ) Hassk . leaf extract : An alternative approach for the treatment of staphylococcal bovine mastitis. YRVSC, 102, 242–246.

Ojiako, E. N. (2014). Phytochemical Analysis and Antimicrobial Screening Of Moringa Oleifera Leaves Extract. The International Journal of Engineering And Sciencesournal of Engineering And Sciences, 3(3), 32–35.

Olas, B., Wachowicz, B., Stochmal, A., & Oleszek, W. (2002). Anti-platelet effects of different phenolic compounds from Yucca schidigera Roezl . Bark, 167–173.

Qi, R., Yatomi, Y., & Ozaki, Y. (2001). Effects of incubation time, temperature, and anticoagulants on platelet aggregation in whole blood. Thrombosis Research, 101(3), 139–144.

Rahman, K. (2007). Studies on free radicals, antioxidants, and co-factors. Clinical Interventions in Aging, 2(2), 219–236.

Rosafio, F., Lelli, N., Mimmi, S., Vandelli, L., Bigliardi, G., Dell’Acqua, M. L., … Zini, A. (2017). Platelet Function Testing in Patients with Acute Ischemic Stroke: An Observational Study. Journal of Stroke and Cerebrovascular Diseases, 26(8), 1864–1873.

Salles, I. I., Broos, K., Fontayne, A., Sz??nt??, T., Ruan, C., Nurden, A. T., … Deckmyn, H. (2010). Development of a high-throughput ELISA assay for platelet function testing using platelet-rich plasma or whole blood. Thrombosis and Haemostasis, 104(2), 392–401.

Sambamoorthi, U., Tan, X., & Deb, A. (2015). Multiple chronic conditions and healthcare costs among adults. Expert Review of Pharmacoeconomics & Outcomes Research, 15(5), 823–832.

Sloan, C. L., Sloan, G. J., & Cannon, J. G. (2006). Anti-inflammatory influence of P-selectin on human mononuclear cells. Vascular Pharmacology, 44(3), 166–169.

Voss, R., Morgenstern, E., Waas, W., & Matzdorff, A. (2006). No increase in CD62P-positive single platelets after acute platelet activation in vivo. Thrombosis Research, 117(4), 393–399.

Wu, J., Zhao, M., Wang, Y., Wang, Y., Zhu, H., Zhao, S., & Peng, S. (2016). A novel lead of P-selectin inhibitor: Discovery, synthesis, bioassays and action mechanism. Bioorganic and Medicinal Chemistry Letters, 26(19), 4631–4636.

Wu, P., Ma, G., Li, N., Deng, Q., Yin, Y., & Huang, R. (2015). Investigation of in vitro and in vivo antioxidant activities of flavonoids rich extract from the berries of Rhodomyrtus tomentosa(Ait.) Hassk. Food Chemistry, 173, 194–202.

Zhang, W., Xu, Q., Zhuang, Y., & Chen, Y. (2016). Novel association of soluble intercellular adhesion molecule�1 and soluble P-selectin with the ABO blood group in a Chinese population. Experimental and Therapeutic Medicine, 1–6.



  • There are currently no refbacks.