Impact of Supplementation Rich in CYP2E1 Enzyme and Decarboxylase Enzyme on improvement of Leukocyte Values
DOI:
https://doi.org/10.21834/e-bpj.v10iSI35.7521Keywords:
Benzene, Leukocyte, Enzymes, Occupational HealthAbstract
This study explores the effects of enzyme supplementation (rich in CYP2E1 and decarboxylase) on leukocyte levels among 27 home-based shoe industry workers exposed to benzene in Tambak Osowilangun, Surabaya. Using a quantitative pre-experimental one-group pretest-posttest design, leukocyte counts were assessed before and after five days of supplementation. Results showed a significant improvement, with abnormal leukocyte levels dropping from 59% to 7%. Although limited by a small sample size and lack of a control group, the findings highlight the potential benefits of enzymatic intervention and underscore the need for health-based strategies to reduce occupational risks in informal sectors.
References
Abbas, A. K., Lichtman, A. H., & Pillai, S. (2022). Basic immunology: Functions and disorders of the immune system (6th ed.). Elsevier.
Agency for Toxic Substances and Disease Registry (ATSDR). (2022). Toxicological profile for benzene. U.S. Department of Health and Human Services, Public Health Service. https://wwwn.cdc.gov/TSP/ToxProfiles/ToxProfiles.aspx?id=40&tid=14
Bolt, H. M., & Roos, P. H. (2020). Cytochrome P450 enzymes in the bioactivation of benzene and toluene: Implications for human risk assessment. Toxicology Letters, 331, 11–20. https://doi.org/10.1016/j.toxlet.2020.06.009
Chen, H., et al. (2019). Benzene-induced mouse hematotoxicity is regulated by a protein phosphatase 2A complex that stimulates transcription of cytochrome P450 2E1. Archives of Toxicology, 93, 3219–3231. https://doi.org/10.1007/s00204-019-02583-5
Cordiano, R., Papa, V., Cicero, N., Spatari, G., Allegra, A., & Gangemi, S. (2022). Effects of benzene: Hematological and hypersensitivity manifestations in residents living in oil refinery areas. Toxics, 10(11), 678. https://doi.org/10.3390/toxics10110678
Dietrich, W., & Fowler, S. (2014). Cigarette smoking, oxidative stress, and white blood cell count: Implications for immune function. Journal of Leukocyte Biology, 96(2), 225–234. https://doi.org/10.1189/jlb.3VMA0913-438R
Fang, M., Wang, X., Jia, Z., Qiu, Q., Li, P., Chen, L., & Yang, H. (2022). A simple and efficient method for the substrate identification of amino acid decarboxylases. International Journal of Molecular Sciences, 23(23), 14551. https://doi.org/10.3390/ijms232314551
García Samuelsson, M., Tárraga López, P. J., López-González, Á. A., Paublini, H., Martínez-Almoyna Rifá, E., & Ramírez-Manent, J. I. (2025). Assessment of the risk of insulin resistance in workers classified as metabolically healthy obese. Nutrients, 17(8), 1345. https://doi.org/10.3390/nu17081345
Garcia Ruiz, C., & Fernández Checa, J. C. (2020). Dietary modulation of cytochrome P450 2E1: Implications for oxidative stress and carcinogen metabolism. Nutrition & Metabolism, 17, 48. https://doi.org/10.1186/s12986-020-00482-1
IARC. (2012). IARC Monographs on the Evaluation of Carcinogenic Risks to Humans: Volume 100F, Chemical Agents and Related Occupations. https://publications.iarc.who.int/monographs/ENG/Monographs/vol100F.pdf
Lotsios, N. S., Arvanitis, N., Charonitakis, A. G., Mpekoulis, G., Frakolaki, E., Vassilaki, N., Sideris, D. C., & Vassilacopoulou, D. (2023). Expression of human L-Dopa decarboxylase (DDC) under conditions of oxidative stress. Current Issues in Molecular Biology, 45(12), 10179–10192. https://doi.org/10.3390/cimb45120635
Lv, J., Zhang, C., Liu, X., Gu, C., Liu, Y., Gao, Y., Huang, Z., Jiang, Q., Chen, B., He, D., Wang, T., Xu, Z., & Su, W. (2024). An aging-related immune landscape in the hematopoietic immune system. Immun Ageing, 21, 3. https://doi.org/10.1186/s12979-023-00403-2
Moriguchi, T., & Yamashita, T. (2020). Histamine and histidine decarboxylase: Regulatory mechanisms and immunomodulatory functions. Frontiers in Immunology. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7497259/
National Institute for Occupational Safety and Health. (2008). NIOSH Pocket Guide to Chemical Hazards (3rd ed.). U.S. Department of Health and Human Services. https://www.cdc.gov/niosh/npg/npgd0049.html
Nieman, D. C. (2020). Exercise immunology: Future directions for research related to health and fitness. Journal of Sport and Health Science, 9(3), 281–292. https://doi.org/10.1016/j.jshs.2019.12.002
Penaloza, C. G., Cruz, M., Germain, G., Jabeen, S., Javdan, M., Lockshin, R. A., & Zakeri, Z. (2020). Higher sensitivity of female cells to ethanol: Methylation of DNA lowers Cyp2e1, generating more ROS. Cell Communication and Signaling, 18, 111. https://doi.org/10.1186/s12964-020-00616-8
Rachman, R. F., Tuasikal, I. Z., Tualeka, A. R., Mahmudiono, T., & Suwardi, S. (2022). Evaluation of the exposure to benzene and SpmA using the urine of workers in the shoe home industry in Surabaya. The Indonesian Journal of Occupational Safety and Health, 11(3), 436–444. https://doi.org/10.20473/ijosh.v11i3.2022.436-444
Wang, Y., Guo, X., & Zhou, Y. (2022). Oxidative stress, glutathione, and CYP2E1 in 1,4-dioxane toxicity: Insights into hepatotoxic and hematotoxic mechanisms. Environmental Toxicology and Pharmacology, 95, 10361651. https://pmc.ncbi.nlm.nih.gov/articles/PMC10361651/
Wu, Y., Zhang, J., & Li, Q. (2023). Dietary sources of decarboxylase and their role in immune function. Nutrients, 15(4), 912. https://doi.org/10.3390/nu15040912
Zhang, H., Li, H., Peng, Z., Cao, J., Bao, J., Li, L., Wang, X., Ji, Y., & Chen, Z. (2022). Meta-analysis of the effect of low-level occupational benzene exposure on human peripheral blood leukocyte counts in China. Journal of environmental sciences (China), 114, 204–210. https://doi.org/10.1016/j.jes.2021.08.035
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