Human Behaviour and Responses Challenge towards Emergence of Infectious Diseases: E.coli clinical isolate


  • Ummi Mohlisi Mohd Asmawi
  • Mohammad Nazmul Hasan Maziz
  • Mohammad Abdur Rashid
  • Jamal Houssaini



Consumption of undercooked ground beef is the most common route of transmission of verotoxin-producing E.coli. It is estimated that non-O157 verotoxigenic E.coli (VTEC) can cause diarrhea.The sample was isolated from Universiti Malaya Medical Centre. All the isolates were identified using agarose gel electrophoresis method. This study aims to detect the verotoxin genes and detect the link or involvement of plasmids with these verotoxin genes.  Therefore, this study will contribute to shed new light on resolving the significant and global problem of diarrheal disease caused by this particular pathogenic organism and help in improvising novel therapeutic approaches to improve human healthcare.

© 2016. The Authors. Published for AMER ABRA by e-International Publishing House, Ltd., UK. Peer–review under responsibility of AMER (Association of Malaysian Environment-Behaviour Researchers), ABRA (Association of Behavioural Researchers on Asians) and cE-Bs (Centre for Environment-Behaviour Studies, Faculty of Architecture, Planning & Surveying, Universiti Teknologi MARA, Malaysia.

Keywords: E.coli; non-0157; plasmid profile


Aiij, C., & Borst, P. (1972). The gel electrophoresis of DNA. Biochimica et Biophysica Acta. 269, 197-200.

Áine M., Brian B., Séamus F., Torres S., David M., & Declan J.B. (2011). Serotypes and Virulence Profiles of Non-O157 Shiga Toxin-Producing Escherichia coli Isolates from Bovine Farms. Applied and environmental microbiology 77(24): 8662–8668.

Bettelheim, K.A. (2000). Role of non-O157 VTEC. Journal ofapplied microbiology symposium supplement 29(38); 38S-50S.

Bokete, T.N., O’Callahan, C.M., Clausen, C.R., Tang, N.M., Tran, N., Moseley, S.L., Fitsche, T.R., & Tarr, P.I. (1993). Shiga-like toxin-producing Escherichia coli in Seattle children: a prospective study. Gastroenterology 105, 1724-1731.

Bratoeva, M.P., John, J.F., & Barg, N.L. (1992). Molecular epidemiology of trimethoprim-resistant Shigella boydii serotype 2 strains from Bulgaria. Journal of clinical microbiology 30, 1428-1431.

Fernandes, R.M., Ramos, S.R. Rassi, V., Blake, P.A., & Gomes, T.A. (1992). Use of plasmid profiles to differentiate strains within specific serotypes of classical enteropathogenic Escherichia coli. Brazilian journal of medical and biology research 25(7), 667-672.

Food Safety Inspection Services. 2015. Assessed online at

Joseph M.B., & Mohammad, K. (2012). Predicting the Presence of Non-O157 Shiga Toxin- Producing Escherichia coli in Ground Beef by Using Molecular Tests for Shiga Toxins, Intimin, and O Serogroups. Applied and environmental microbiology 78(19), 7152–7155.

Liao, Y.T., Miller, M.F., Loneragan, G.H., Brooks, J.C., Echeverry, A., & Brashears, M.M. (2014). Non-O157 Shiga toxin-producing Escherichia coli in U. S. retail ground beef. Journal of food protection 77(7),1188-92.

Liorente, P., Barnech, L., Irino, K., Rumi, M.V., & Bentancor, A. (2014). Characterization of Shiga toxin-producing Escherichia coli isolated from ground beef collected in different socioeconomic strata markets in Buenos Aires, Argentina. BioMed research international 2014(795104), 1-9.

Ma, J., Mark Ibekwe, A., Crowley, D.E., & Yang, C.H. (2014). Persistence of Escherichia coli O157 and non-O157 strains in agricultural soils. Science of The total environment 490,822-829.

Misselwitz, J., Karch, H., Bielazewska, M., John, U., Ringelmann, F., Ronnefarth, G., & Patzer, L. (2003). Cluster of hemolytic-uremic syndrome caused by Shiga toxin-producing Escherichia coli O26:H11. The pediatric infectious disease journal 22(4), 349-354.

Nataro, J.P., & Kaper, J.B (1998). Diarrheagenic Escherichia coli. Clinical microbiology reviews 11,142-201.

Nielsen, E.M., & Andersen, M.T. (2003). Detection and characterization of verocytotoxin-producing Escherichia coli by automated 5' nuclease PCR assay. Journal of clinical microbiology 41(7), 2884-2893.

Niu, Y.D., McAllister, T.A., Nash, J.H., Kropinski, A.M., & Stanford, K. (2014). Four Escherichia coli O157:H7 phages: a new bacteriophage genus and taxonomic classification of T1-like phages. PLoS One. 9(6), e100426. 1-11.

Polifroni, R., Etcheverría, A.I., Arroyo, G.H., & Padola, N.L. (2014). Survival of VTEC O157 and non-O157 in water troughs and bovine feces. Revista Argentina de microbiologia 46(2),126-132.

Rajkhowa, S., & Sarma, D.K. (2014). Prevalence and antimicrobial resistance of porcine O157 and non-O157 Shiga toxin-producing Escherichia colifrom India. Tropical animal health production 46(6):931-937.

Schmidt, H., Geitz, C., Tarr, P.I., Frosch, M., & Karch, H. (1999). Non-O157:H7 pathogenic Shiga toxin-producing Escherichia coli: phenotypic and genetic profiling of virulence traits and evidence for clonality. Journal of infectious diseases 179(1), 115-123.

Wan Himratul, A. (1999). Detection and molecular analysis of non-O157 diarrheagenic Escherichia coli isolates by using VT1 probe. (Master’s thesis, University of Malaya, Malaysia).



How to Cite

Mohd Asmawi, U. M., Maziz, M. N. H., Abdur Rashid, M., & Houssaini, J. (2016). Human Behaviour and Responses Challenge towards Emergence of Infectious Diseases: E.coli clinical isolate. Environment-Behaviour Proceedings Journal, 1(1), 146-151.