Effectiveness of Celastrol in Reducing Inflammatory Cell Infiltration via H&E in the Liver of High-Fat Diet-Fed ApoE-Knockout Mice

Arifah Ahmad Damahuri; Thuhairah Hasrah Abdul Rahman; Yakoh Karemdabeh; Nasibah Azme

doi:10.21834/e-bpj.v10iSI35.7505

Authors

Arifah Ahmad Damahuri Institute of Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh, Malaysia; Laboratory Animal Care Unit, Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh, Malaysia
Thuhairah Hasrah Abdul Rahman Department of Pathology, Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh, Malaysia
Yakoh Karemdabeh Department of Chemistry, Faculty of Education, Fatoni University, Yala, Thailand
Nasibah Azme Laboratory Animal Care Unit, Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh, Malaysia ; Department of Physiology, Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh, Malaysia

DOI:

https://doi.org/10.21834/e-bpj.v10iSI35.7505

Keywords:

Celastrol, Inflammatory cell, H&E staining, ApoE-knockout mice

Abstract

High-fat diet (HFD) promotes liver inflammation by increasing infiltration of inflammatory cells. This study analyzed the effect of celastrol on hepatic inflammation in ApoE-knockout mice on HFD. Treatment with celastrol (1.5, 2, or 2.5 mg/kg/day) for 4 weeks, followed by liver tissue staining with H&E. Quantification of infiltration areas was obtained with NDP.view 2 software and analyzed with the Kruskal-Wallis test. At the maximum dose (2.5 mg/kg), the inflammatory cell infiltration of the liver was significantly decreased in comparison with untreated HFD controls. These results justify the potential use of celastrol as an anti-inflammatory in diet-induced liver inflammation.

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